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vantictumab ma5-42006  (Fisher Scientific)
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Modulation of WNT signaling controls AF closure. (A-D) Wholemount Alizarin Red and Alcian Blue staining at E18.5 and P5 showing that Scx-Cre;Ctnnb1 GOF mice exhibit a wide-open AF that fails to close compared to littermate controls. n =3 littermate pairs per stage. (E-G) A WNT inhibitor cocktail composed of recombinant mouse WIF1 protein and <t>vantictumab</t> was administered at P3 through a single injection under the scalp in the area above the AF. (E) Wholemount Alizarin Red and Alcian Blue staining of a treated control collected at P7 showed that the treatment had no negative effects on calvarial bone formation and AF closure. (F) Wholemount skeletal stains of three treated Wnt1-Cre;Fgfr2 −/− littermates showed that AF closure is partially restored. (G) Quantification of the AF area at P7 in control and Wnt1-Cre;Fgfr2 −/− mice that were treated (Tx) with WIF1/vantictumab at P3 or untreated.
Vantictumab Ma5 42006, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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influenza b virus, b/brisbane/33/2008 (victoria lineage) nr-42006  (BEI Resources)
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Modulation of WNT signaling controls AF closure. (A-D) Wholemount Alizarin Red and Alcian Blue staining at E18.5 and P5 showing that Scx-Cre;Ctnnb1 GOF mice exhibit a wide-open AF that fails to close compared to littermate controls. n =3 littermate pairs per stage. (E-G) A WNT inhibitor cocktail composed of recombinant mouse WIF1 protein and <t>vantictumab</t> was administered at P3 through a single injection under the scalp in the area above the AF. (E) Wholemount Alizarin Red and Alcian Blue staining of a treated control collected at P7 showed that the treatment had no negative effects on calvarial bone formation and AF closure. (F) Wholemount skeletal stains of three treated Wnt1-Cre;Fgfr2 −/− littermates showed that AF closure is partially restored. (G) Quantification of the AF area at P7 in control and Wnt1-Cre;Fgfr2 −/− mice that were treated (Tx) with WIF1/vantictumab at P3 or untreated.
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Modulation of WNT signaling controls AF closure. (A-D) Wholemount Alizarin Red and Alcian Blue staining at E18.5 and P5 showing that Scx-Cre;Ctnnb1 GOF mice exhibit a wide-open AF that fails to close compared to littermate controls. n =3 littermate pairs per stage. (E-G) A WNT inhibitor cocktail composed of recombinant mouse WIF1 protein and <t>vantictumab</t> was administered at P3 through a single injection under the scalp in the area above the AF. (E) Wholemount Alizarin Red and Alcian Blue staining of a treated control collected at P7 showed that the treatment had no negative effects on calvarial bone formation and AF closure. (F) Wholemount skeletal stains of three treated Wnt1-Cre;Fgfr2 −/− littermates showed that AF closure is partially restored. (G) Quantification of the AF area at P7 in control and Wnt1-Cre;Fgfr2 −/− mice that were treated (Tx) with WIF1/vantictumab at P3 or untreated.
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Modulation of WNT signaling controls AF closure. (A-D) Wholemount Alizarin Red and Alcian Blue staining at E18.5 and P5 showing that Scx-Cre;Ctnnb1 GOF mice exhibit a wide-open AF that fails to close compared to littermate controls. n =3 littermate pairs per stage. (E-G) A WNT inhibitor cocktail composed of recombinant mouse WIF1 protein and <t>vantictumab</t> was administered at P3 through a single injection under the scalp in the area above the AF. (E) Wholemount Alizarin Red and Alcian Blue staining of a treated control collected at P7 showed that the treatment had no negative effects on calvarial bone formation and AF closure. (F) Wholemount skeletal stains of three treated Wnt1-Cre;Fgfr2 −/− littermates showed that AF closure is partially restored. (G) Quantification of the AF area at P7 in control and Wnt1-Cre;Fgfr2 −/− mice that were treated (Tx) with WIF1/vantictumab at P3 or untreated.
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Modulation of WNT signaling controls AF closure. (A-D) Wholemount Alizarin Red and Alcian Blue staining at E18.5 and P5 showing that Scx-Cre;Ctnnb1 GOF mice exhibit a wide-open AF that fails to close compared to littermate controls. n =3 littermate pairs per stage. (E-G) A WNT inhibitor cocktail composed of recombinant mouse WIF1 protein and <t>vantictumab</t> was administered at P3 through a single injection under the scalp in the area above the AF. (E) Wholemount Alizarin Red and Alcian Blue staining of a treated control collected at P7 showed that the treatment had no negative effects on calvarial bone formation and AF closure. (F) Wholemount skeletal stains of three treated Wnt1-Cre;Fgfr2 −/− littermates showed that AF closure is partially restored. (G) Quantification of the AF area at P7 in control and Wnt1-Cre;Fgfr2 −/− mice that were treated (Tx) with WIF1/vantictumab at P3 or untreated.
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Modulation of WNT signaling controls AF closure. (A-D) Wholemount Alizarin Red and Alcian Blue staining at E18.5 and P5 showing that Scx-Cre;Ctnnb1 GOF mice exhibit a wide-open AF that fails to close compared to littermate controls. n =3 littermate pairs per stage. (E-G) A WNT inhibitor cocktail composed of recombinant mouse WIF1 protein and <t>vantictumab</t> was administered at P3 through a single injection under the scalp in the area above the AF. (E) Wholemount Alizarin Red and Alcian Blue staining of a treated control collected at P7 showed that the treatment had no negative effects on calvarial bone formation and AF closure. (F) Wholemount skeletal stains of three treated Wnt1-Cre;Fgfr2 −/− littermates showed that AF closure is partially restored. (G) Quantification of the AF area at P7 in control and Wnt1-Cre;Fgfr2 −/− mice that were treated (Tx) with WIF1/vantictumab at P3 or untreated.
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cannabidivarin gwp-42006  (GW Pharmaceuticals)
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Modulation of WNT signaling controls AF closure. (A-D) Wholemount Alizarin Red and Alcian Blue staining at E18.5 and P5 showing that Scx-Cre;Ctnnb1 GOF mice exhibit a wide-open AF that fails to close compared to littermate controls. n =3 littermate pairs per stage. (E-G) A WNT inhibitor cocktail composed of recombinant mouse WIF1 protein and <t>vantictumab</t> was administered at P3 through a single injection under the scalp in the area above the AF. (E) Wholemount Alizarin Red and Alcian Blue staining of a treated control collected at P7 showed that the treatment had no negative effects on calvarial bone formation and AF closure. (F) Wholemount skeletal stains of three treated Wnt1-Cre;Fgfr2 −/− littermates showed that AF closure is partially restored. (G) Quantification of the AF area at P7 in control and Wnt1-Cre;Fgfr2 −/− mice that were treated (Tx) with WIF1/vantictumab at P3 or untreated.
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Modulation of WNT signaling controls AF closure. (A-D) Wholemount Alizarin Red and Alcian Blue staining at E18.5 and P5 showing that Scx-Cre;Ctnnb1 GOF mice exhibit a wide-open AF that fails to close compared to littermate controls. n =3 littermate pairs per stage. (E-G) A WNT inhibitor cocktail composed of recombinant mouse WIF1 protein and vantictumab was administered at P3 through a single injection under the scalp in the area above the AF. (E) Wholemount Alizarin Red and Alcian Blue staining of a treated control collected at P7 showed that the treatment had no negative effects on calvarial bone formation and AF closure. (F) Wholemount skeletal stains of three treated Wnt1-Cre;Fgfr2 −/− littermates showed that AF closure is partially restored. (G) Quantification of the AF area at P7 in control and Wnt1-Cre;Fgfr2 −/− mice that were treated (Tx) with WIF1/vantictumab at P3 or untreated.

Journal: Development (Cambridge, England)

Article Title: FGFR2 directs inhibition of WNT signaling to regulate anterior fontanelle closure during skull development

doi: 10.1242/dev.204264

Figure Lengend Snippet: Modulation of WNT signaling controls AF closure. (A-D) Wholemount Alizarin Red and Alcian Blue staining at E18.5 and P5 showing that Scx-Cre;Ctnnb1 GOF mice exhibit a wide-open AF that fails to close compared to littermate controls. n =3 littermate pairs per stage. (E-G) A WNT inhibitor cocktail composed of recombinant mouse WIF1 protein and vantictumab was administered at P3 through a single injection under the scalp in the area above the AF. (E) Wholemount Alizarin Red and Alcian Blue staining of a treated control collected at P7 showed that the treatment had no negative effects on calvarial bone formation and AF closure. (F) Wholemount skeletal stains of three treated Wnt1-Cre;Fgfr2 −/− littermates showed that AF closure is partially restored. (G) Quantification of the AF area at P7 in control and Wnt1-Cre;Fgfr2 −/− mice that were treated (Tx) with WIF1/vantictumab at P3 or untreated.

Article Snippet: Stock vantictumab (Fisher Scientific, MA5-42006) and recombinant WIF1 protein (R&D Systems, 135-WF-050) were diluted in sterile water to a final concentration of 1.1 mg/ml and 0.5 mg/ml, respectively.

Techniques: Staining, Recombinant, Injection, Control